منابع مشابه
Cre-ating Ways to Serotonin
Almost 50 years ago Dahlström and Fuxe discovered serotonergic neurons in the brainstem. Much remains to learn, however, about how serotonin (5hydroxytryptamine or 5HT) modulates circuit activity. Serotonergic neurons release 5HT throughout the CNS. Accordingly, these cells influence breathing rhythms, body temperature, heart rate, pain, eating, sleep, arousal, emotion, and cognition to list a ...
متن کاملCre-ating Somatic Cell Genetic Mosaics in the Mouse
Generation of somatic mosaics in which mutant cell clones are uniquely and completely labeled has yielded considerable insight into many biological processes in Drosophila. In this issue of Cell, describe a novel method called MADM that allows the generation of such mosaics in mice.
متن کاملTrapping of mammalian promoters by Cre-lox site-specific recombination.
One of the challenges in human genome research is to identify the promoter sequences which play a key role in the regulation of gene expression. We report here a new promoter trapping system for use with mammalian cells comprised of the following three steps: 1) Cloning of DNA fragments into a promotertrapping vector, 2) integration of the trapping vector into a designated target in the mammali...
متن کاملThe conditional KO approach: Cre/Lox technology in human neurons
The use of human pluripotent stem cells to model human diseases has become a new standard in biomedical sciences. To this end, patient-derived somatic cells are studied in vitro to mimic human pathological conditions. Here, we describe an alternative experimental strategy, the 'conditional KO approach', which allows engineering disease-relevant mutations in pluripotent stem cells from healthy d...
متن کاملModular Cre/lox System and Genetic Therapeutics for Colorectal Cancer
The Cre/lox system is a powerful tool for targeting therapeutic effectors in a wide variety of human disorders. I review a Cre/lox Wnt-targeted system that has shown promise against Wnt-positive colorectal cancer cell lines. In addition to Wnt-specific targeting of cell death inducers, the modular nature of this gene therapy model system can be exploited by designing positive and negative feedb...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: EMBO reports
سال: 2000
ISSN: 1469-221X,1469-3178
DOI: 10.1093/embo-reports/kvd035